Journal: PLoS ONE

Article Title: Association of genetic variations in ACE2 , TIRAP and factor X with outcomes in COVID-19

doi: 10.1371/journal.pone.0260897

Figure Lengend Snippet: Abbreviations: IL; interleukin, MyD88; myeloid differentiation factor 88, NFkB; nuclear factor-kappa B, TIRAP; toll/interleukin-1 receptor domain-containing adaptor protein, TLR; toll-like receptor, TRAF6; TNF Receptor Associated Factor 6, TRIF; TIR-domain-containing adapter-inducing interferon-β, ssRNA; single-strand ribonucleic acid, dsRNA; double-strand ribonucleic acid.

Article Snippet: For the ACE2 and TIRAP SNPs, real time PCRs with specific primer pairs and probes (Biolegio Lab Equipment B.V., Nijmegen, the Netherlands) were performed to determine the various alleles, see for primers and probes sequences.

Techniques:

Journal: PLoS ONE

Article Title: Association of genetic variations in ACE2 , TIRAP and factor X with outcomes in COVID-19

doi: 10.1371/journal.pone.0260897

Figure Lengend Snippet: ACE2 and TIRAP primers and probes sequences.

Article Snippet: For the ACE2 and TIRAP SNPs, real time PCRs with specific primer pairs and probes (Biolegio Lab Equipment B.V., Nijmegen, the Netherlands) were performed to determine the various alleles, see for primers and probes sequences.

Techniques:

Journal: PLoS ONE

Article Title: Association of genetic variations in ACE2 , TIRAP and factor X with outcomes in COVID-19

doi: 10.1371/journal.pone.0260897

Figure Lengend Snippet: Genotype frequencies in survivors and non-survivors.

Article Snippet: For the ACE2 and TIRAP SNPs, real time PCRs with specific primer pairs and probes (Biolegio Lab Equipment B.V., Nijmegen, the Netherlands) were performed to determine the various alleles, see for primers and probes sequences.

Techniques:

Journal: PLoS ONE

Article Title: Association of genetic variations in ACE2 , TIRAP and factor X with outcomes in COVID-19

doi: 10.1371/journal.pone.0260897

Figure Lengend Snippet: Genotype frequencies in patients with and without venous thromboembolism.

Article Snippet: For the ACE2 and TIRAP SNPs, real time PCRs with specific primer pairs and probes (Biolegio Lab Equipment B.V., Nijmegen, the Netherlands) were performed to determine the various alleles, see for primers and probes sequences.

Techniques:

Journal: PLoS ONE

Article Title: Association of genetic variations in ACE2 , TIRAP and factor X with outcomes in COVID-19

doi: 10.1371/journal.pone.0260897

Figure Lengend Snippet: Biochemical parameters in patients with TIRAP carriage and TIRAP wild-type.

Article Snippet: For the ACE2 and TIRAP SNPs, real time PCRs with specific primer pairs and probes (Biolegio Lab Equipment B.V., Nijmegen, the Netherlands) were performed to determine the various alleles, see for primers and probes sequences.

Techniques: Significance Assay

Journal: PLoS ONE

Article Title: Association of genetic variations in ACE2 , TIRAP and factor X with outcomes in COVID-19

doi: 10.1371/journal.pone.0260897

Figure Lengend Snippet: Genotype frequencies in patients with and without respiratory failure.

Article Snippet: For the ACE2 and TIRAP SNPs, real time PCRs with specific primer pairs and probes (Biolegio Lab Equipment B.V., Nijmegen, the Netherlands) were performed to determine the various alleles, see for primers and probes sequences.

Techniques:

Journal: PLoS ONE

Article Title: 16S rRNA Gene Sequence-Based Identification of Bacteria in Automatically Incubated Blood Culture Materials from Tropical Sub-Saharan Africa

doi: 10.1371/journal.pone.0135923

Figure Lengend Snippet: PCR protocols used for this study.

Article Snippet: Two different, previously described SybrGreen 16S rRNA gene real-time PCRs were performed from the purified samples on a Rotor Gene 6000 real-time rotary analyzer (Corbett Life Science, Qiagen, Hamburg, Germany).

Techniques: SYBR Green Assay, Concentration Assay, Amplification

Journal: PLoS ONE

Article Title: 16S rRNA Gene Sequence-Based Identification of Bacteria in Automatically Incubated Blood Culture Materials from Tropical Sub-Saharan Africa

doi: 10.1371/journal.pone.0135923

Figure Lengend Snippet: Summarized results of cultural growth and 16S rRNA gene PCR.

Article Snippet: Two different, previously described SybrGreen 16S rRNA gene real-time PCRs were performed from the purified samples on a Rotor Gene 6000 real-time rotary analyzer (Corbett Life Science, Qiagen, Hamburg, Germany).

Techniques:

Journal: PLoS ONE

Article Title: 16S rRNA Gene Sequence-Based Identification of Bacteria in Automatically Incubated Blood Culture Materials from Tropical Sub-Saharan Africa

doi: 10.1371/journal.pone.0135923

Figure Lengend Snippet: Results of 917 bp 16S rRNA gene sequencing and consecutive 357 bp 16S rRNA gene sequencing in case of negative 917 bp 16S rRNA gene PCR results of culture-positive samples.

Article Snippet: Two different, previously described SybrGreen 16S rRNA gene real-time PCRs were performed from the purified samples on a Rotor Gene 6000 real-time rotary analyzer (Corbett Life Science, Qiagen, Hamburg, Germany).

Techniques: Sequencing

Journal: PLoS ONE

Article Title: 16S rRNA Gene Sequence-Based Identification of Bacteria in Automatically Incubated Blood Culture Materials from Tropical Sub-Saharan Africa

doi: 10.1371/journal.pone.0135923

Figure Lengend Snippet: Results of 917 bp 16S rRNA gene sequencing of culture-positive samples.

Article Snippet: Two different, previously described SybrGreen 16S rRNA gene real-time PCRs were performed from the purified samples on a Rotor Gene 6000 real-time rotary analyzer (Corbett Life Science, Qiagen, Hamburg, Germany).

Techniques: Sequencing

Journal: PLoS ONE

Article Title: 16S rRNA Gene Sequence-Based Identification of Bacteria in Automatically Incubated Blood Culture Materials from Tropical Sub-Saharan Africa

doi: 10.1371/journal.pone.0135923

Figure Lengend Snippet: Results of the 917 bp 16S rRNA gene PCR and the 357 bp 16S rRNA gene PCR from culture-negative samples.

Article Snippet: Two different, previously described SybrGreen 16S rRNA gene real-time PCRs were performed from the purified samples on a Rotor Gene 6000 real-time rotary analyzer (Corbett Life Science, Qiagen, Hamburg, Germany).

Techniques: Environmental Sampling, Sample Prep